Techniques for Molecular Biology

Front Cover
Science Publishers, Jan 4, 2006 - 227 pages
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With the underpinning role of forage legumes in the nitrogen economy and animal productivity from temperate grasslands certain to expand in the future, particularly in regions where their potential has not yet been realized, it is essential that the wealth of information currently available is widely disseminated. This book serves the purpose with very detailed information on and illustrations of 35 selected forage legume species that will contribute to more efficient and viable grassland farming.
 

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IT IS ONE OF THE GOOD BOOK I HAD EVER GO THROUGH FOR THE REFERENCES AND TO UNDERSTAND THE BACKGROUND OF A TECHNIQUE.
THANKS

Contents

Structure and Expression of a Eukaryote Gene Coding for an mRNA and a Protein
3
Parameters of Description of a Genome
5
Sequencing of Whole Genomes
9
Vectors and Cloning
13
Restriction Enzymes
15
Electrophoresis of Nucleic Acids
19
Description of a Plasmid and a Phagemid
21
Description of a Bacteriophage and a Cosmid
23
Determination of Transcription Initiation Site
111
Functional Analysis of Promoters
113
Gel Retardation
117
DNase I Footprinting
119
Genetic Transformation of Eukaryotes
121
Generic Transformation of Plants by Agrobacterium tumefaciens
123
Direct Gene Transfer into Plant Protoplasts
129
Direct Gene Transfer by Biolistics
131

Description of a YAC and Other Large Vectors
25
Molecular Cloning
27
Genetic Transformation of Bacteria and Yeasts
31
Labelling of Nucleic Acids and Hybridization
33
DNA Labelling
35
Molecular Hybridization
39
In situ Hybridization of mRNA
43
DNA Libraries and Screening
47
Construction of a Genomic DNA Library
49
Construction of a cDNA Library
51
Screening of a Library
53
Subtractive Libraries AFLPcDNA
57
Differential Display RTPCR
61
Suppression Subtractive
65
Representational Difference Analysis
69
Expressed Sequence Tags
73
DNA Microchips cDNA Filters
77
Characterization of a Gene
89
DNA Sequencing
91
Polymerase Chain Reaction
97
Rapid Amplification of cDNA Ends
99
Genome Walking by PCR
103
Reverse Transcriptase PCR
105
In vitro Transcription
109
Genetic Transformation of Animal Cells
133
Cloning of Animals
137
Transient Expression
141
Analysis of Gene Function
143
Recombinant Proteins
145
Baculoviruses of Insects Vectors of Expression of Foreign Genes
149
Yeast two Hybrid System
155
SiteDirected Mutagenesis
161
Mutation Complementation in Yeast
167
Knockout of Genes in Yeast
171
Gene Tagging
175
RNA Interference
181
Polymorphism of a Genome
185
Molecular Markers
187
Genetic and Physical Maps
193
Pulse Field Gel Electrophoresis
197
Restriction Fragment Length Polymorphism
201
Random Amplified Polymorphic DNA
203
Amplified Fragment Length Polymorphism
205
Retromarkers
207
Single Strand Conformation Polymorphism
213
Denaturing Gel Gradient Electrophoresis
215
List of Contributors
225
Copyright

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